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CCK与海人酸诱发癫痫敏感性形成的关系

来源:解剖科学进展
摘要:摘要:用海人酸(Kainicacid,KA)10mg/kg给Sprague-Dawley(SD)大鼠颈部皮下注射,诱发急性癫痫发作,在急性癫痫发作后一周,用阈下剂量(5mg/kg)的KA检测癫痫敏感性。同时分别用原位杂交和免疫组化技术检测并发现癫痫敏感性形成大鼠额叶皮层胆囊收缩素原(PCCK)mRNA明显增加。海马结构中,靠近海马齿状回颗粒细胞(DGCs)基底......

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  摘 要:用海人酸(Kainic acid,KA)10mg/kg给Sprague-Dawley(SD)大鼠颈部皮下注射,诱发急性癫痫发作,在急性癫痫发作后一周,用阈下剂量(5mg/kg)的KA检测癫痫敏感性。同时分别用原位杂交和免疫组化技术检测并发现癫痫敏感性形成大鼠额叶皮层胆囊收缩素原(PCCK)mRNA明显增加;海马结构中,靠近海马齿状回颗粒细胞(DGCs)基底部锥体样胆囊收缩素(Cholecystoklnin,CCK)免疫反应阳性神经元染色明显增强,但海马门尖部CCK免疫反应阳性神经元明显减少。而癫痫敏感性未形成大鼠未见上述变化,提示上述变化与癫痫敏感性形成有关。

The Relationship Between CCK and Seizure Susceptibility

wu Yue,Zhang Wanqin(Department of Physiology, Dalian Medical University, Dalian, 116027)

  Abstract:Kainic acid (KA) 10mg/kg was subcutaneously injected to sprague-Dawley rats and induced acute convulsions. A week later, subconvulsive dose of KA (5mg/kg) was used to detect the seizure susceptibility. At the same time, by immunocytochemistry and hybridization, PCCK mRNA was found increased significantly in frontal cortex of susceptible rats. The CCK-IR was enhanced in pyramidal cells near the DGCs of hippocampus and was decreased obviously in the deep region of hilus. These changes were not observed in nonsusceptible rats, which may be relevant to development of seizure susceptibility.

  Keywords:kainic acid; frontal cortex; hippocampus; seizure susceptibility; CCK参考文献:

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作者: 吴钺 张万琴 2004-9-29
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