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大鼠脑线粒体DNA提取方法

来源:解剖科学进展
摘要:摘要:本研究用一种简便方法提取大鼠脑线粒体DNA(mtDNA),所得的线粒体DNA纯度为1.8±0.01,得率为每克脑组织0.8~1.0μg线粒体DNA,以此为模板进行PCR反应,扩增线粒体DNA编码基因,产量丰富、特异性高。IsolationoftheMitochondrialDNAfromRatBrainxiangPing,GaoGuoquan,YaoZhibin(DepartmentofAnato......

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  摘 要:本研究用一种简便方法提取大鼠脑线粒体DNA(mtDNA),所得的线粒体DNA纯度为1.8±0.01,得率为每克脑组织0.8~1.0μg线粒体DNA,以此为模板进行PCR反应,扩增线粒体DNA编码基因,产量丰富、特异性高。该方法操作简便、成本低,适用于一般实验室。

Isolation of the Mitochondrial DNA from Rat Brain

xiang Ping,Gao Guoquan,Yao Zhibin(Department of Anatomy, Bengbu Medical College, Bengbu, 233003)

  Abstract:A simple technique for isolation of mitochondrial DNA from rat brain is described. The purity (260/280) of mtDNA extracted by the method is 1.8±0. 01, the mtDNA yield per gram brain is 0. 8-10/μg. The product of the PCR is specific. The method can be used in general laboratory because it costs very low and is easy to operate.

  Keywords:mtDNA; rat; PCR参考文献:

  [1]Bibb JM, Van Etten RA, Wright CT, et al. Sequence and gene organization of mouse mitochondrial DNA. Cell, 1981,26:167

  [2]Schon EA, Rizzuto R, Moreas CT, et al. A direct repeat is ahot-spot for large-scale deletion of human mltochondrial dNA.Science, 1989,244: 346

  [3]吴鹤龄,林锦湖.遗传实验方法.北京,高等教育出版社,1983,108-114

  [4]Gadaleta P, Pepe G, Candia D, et al. The complete nuclueotide sequence of the rattus norvegicus mitochondrial geneome : cryptic signal revealed by comparative analysis between vertebrated. J Mol Evol, 1989,28:497

  [5]江谦.现代医学实验方法.北京,人民卫生出版社,1997,335-350

作者: 项平高国全姚志彬 2004-9-30
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