医源世界
首页药品天地专业药学实验技术基本实验技术生物分子定量

Protein concentration of Laemmli gel samples

来源:网络
摘要:ProteinconcentrationofLaemmligelsamplesTo10µ。lboiledlysate(inLaemmlisamplebuffer)add40µ。...

点击显示 收起

 

Protein concentration of Laemmli gel samples


To 10 µl boiled lysate (in Laemmli sample buffer) add 40µl water + 50µl 50% TCA. Ppt. 10 min. on ice. Spin and wash with 10% TCA. Resuspend pellet in 100 µl 1M NaOH, add 900 µl water and assay 100 µl by Bradford method.
 

作者: 佚名 2008-2-3
医学百科App—中西医基础知识学习工具
  • 相关内容
  • 近期更新
  • 热文榜
  • 医学百科App—健康测试工具